IL‐10 from plasmacytoid dendritic cells promotes angiogenesis in the early stage of endometriosis

• DOI: 10.1002/path.5339
• Publication Date: 2019-08-16T13:13:23Z
• Authors: Jau‐Ling Suen; Yu Chang; Yu‐Shiang Shiu; Chia‐Yi Hsu; Pooja Sharma; Chien‐Chih Chiu; Yi‐Ju Chen; Tzyh‐Chyuan Hour; Eing‐Mei Tsai
• Keywords: Adult; Mice, Knockout; 0303 health sciences; Neovascularization, Pathologic; Endometriosis; Interleukin-3 Receptor alpha Subunit; Mice, Nude; Apoptosis; Dendritic Cells; Middle Aged; Original Papers; Adoptive Transfer; Coculture Techniques; Interleukin-10; Mice, Inbred C57BL; Disease Models, Animal; Endometrium; 03 medical and health sciences; Human Umbilical Vein Endothelial Cells; Animals; Humans; Female; Cells, Cultured

An elevated level of IL-10 has been considered a critical factor for the development endometriosis; however, its detailed mechanism and causal relationship remain unclear. This study explored cellular source angiogenic activity local during early stage endometriosis. Using surgical murine model, we found that localised treatment with exogenous recombinant on day surgery significantly enhanced endometriotic lesion growth angiogenesis, whereas blocking using mAbs suppressed those effects. Adoptive transfer Il10+/+ plasmacytoid dendritic cells into mice development, Il10-/- inhibited development. Furthermore, in vitro angiogenesis analyses demonstrated receptor pathway stimulated migratory tube formation ability HUVECs as well ectopic endometrial mesenchymal stem through, at least part, VEGF-dependent pathway. We also directly based Matrigel plug assay zebrafish model. Pathological results from human endometrioma tissues showed increased infiltration CD123+ higher percentages express CD31 compared corresponding normal counterparts. Taken together, these show secreted promotes endometriosis through pathological disease stage. provides scientific basis potential therapeutic strategy targeting IL-10-IL-10 milieu. © 2019 The Authors. Journal Pathology published by John Wiley & Sons Ltd behalf Society Great Britain Ireland.