The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate‐washed membrane standard

• DOI: 10.1002/pmic.201000126
• Publication Date: 2010-05-18T20:08:07Z
• Authors: Lifeng Peng; Eugene A. Kapp; David Fenyö; Min‐Seok Kwon; Pu Jiang; Songfeng Wu; Ying Jiang; Marie‐Isabel Aguilar; Nikhat Ahmed; Mark S. Baker; Zongwei Cai; Yu‐Ju Chen; Phan Van Chi; Maxey C. M. Chung; Fuchu He; Alice C. L. Len; Pao‐Chi Liao; Kazuyuki Nakamura; Sai Ming Ngai; Young‐Ki Paik; Tai‐Long Pan; Terence C. W. Poon; Ghasem Hosseini S...; Richard J. Simpson; Ravi Sirdeshmukh; Chantragan Srisomsap; Jisnuson Svasti; Yu‐Chang Tyan; Florian S. Dreyer; Danyl McLauchlan; Pisana Rawson; T. William Jordan
• Keywords: Proteome; Organelle; Human proteome project

The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison strategies for analysis membrane proteomes and discovery proteins. This multilaboratory project is based the subcellular fraction from mouse liver that contains endoplasmic reticulum other organelles. In this study, we present strategy used preparation initial characterization sample, including validation carbonate-washing step enriches integral lipid-anchored Analysis 17 independent data sets five types proteomic workflows in progress.