Rice suspension cultured cells are evaluated as a model system to study salt responsive networks in plants using a combined proteomic and metabolomic profiling approach

• DOI: 10.1002/pmic.201200425
• Publication Date: 2013-05-10T06:52:48Z
• Authors: Dawei Liu; Kristina L. Ford; Ute Roessner; Siria Natera; Andrew M. Cassin; John H. Patterson; Antony Bacic
• Keywords: Metabolic pathway; Proteome

Salinity is one of the major abiotic stresses affecting plant productivity but surprisingly, a thorough understanding salt-responsive networks responsible for sustaining growth and maintaining crop yield remains significant challenge. Rice suspension culture cells (SCCs), single cell type, were evaluated as model system they provide ready source homogenous type avoid complications multicellular tissue types in planta. A combination performance, transcriptional analyses using known salt-induced genes was performed on control 100 mM NaCl cultured to validate biological system. Protein profiling conducted both DIGE- iTRAQ-based proteomics approaches. In total, 106 proteins identified DIGE experiments 521 iTRAQ with 58 common Metabolomic analysis provided insights into developmental changes rice SCCs at metabolite level; 134 metabolites identified, including 30 amines amides, 40 organic acids, sugars, sugar acids alcohols, 21 fatty sterols, 3 miscellaneous compounds. Our results from proteomic metabolomic studies indicate that are extremely complex share some similarities thee cellular responses observed For instance, carbohydrate energy metabolism pathways, redox signaling auxin/indole-3-acetic acid pathways biosynthesis osmoprotectants all salt responsive enabling maintain function under stress condition. These data discussed context our planta salt-responses.