Resourcing, annotating, and analysing synthetic peptides of SARS‐CoV‐2 for immunopeptidomics and other immunological studies

• DOI: 10.1002/pmic.202100036
• Publication Date: 2021-04-03T05:39:25Z
• Authors: Chen Li; Jerico Revote; Sri H. Ramarathinam; Shan Zou Chung; Nathan P. Croft; Katherine E. Scull; Ziyi Huang; Rochelle Ayala; Asolina Braun; Nicole A. Mifsud; Patricia T. Illing; Pouya Faridi; Anthony W. Purcell
• Keywords: Proteome; Peptide vaccine; Human proteome project

SARS-CoV-2 has caused a significant ongoing pandemic worldwide. A number of studies have examined the T cell mediated immune responses against SARS-CoV-2, identifying potential epitopes derived from proteome. Such will aid in targets for vaccination and monitoring. In this study, we applied tandem mass spectrometry proteomic techniques to library ∼40,000 synthetic peptides, order generate large dataset peptide MS/MS spectra. On basis, built an online knowledgebase, termed virusMS (https://virusms.erc.monash.edu/), document, annotate analyse these peptides their spectral information. VirusMS incorporates user-friendly interface facilitate searching, browsing downloading database content. Detailed annotations including experimental information, modifications, predicted peptide-HLA (human leukocyte antigen) binding affinities, data, are provided virusMS.