Nano‐Ruby: A Promising Fluorescent Probe for Background‐Free Cellular Imaging

• DOI: 10.1002/ppsc.201200112
• Publication Date: 2013-02-27T10:33:46Z
• Authors: Andrew M. Edmonds; Mushtaq A. Sobhan; Varun K. A. Sreen...; Ekaterina A. Greb...; James R. Rabeau; Ewa M. Goldys; Andrei V. Zvyagin
• Keywords: Biomolecule; Autofluorescence; Biological Imaging

Bioprobes based on fluorescent ruby nanoparticles, which are suitable for ultrasensitive imaging, reported. A stable aqueous/buffer colloid, permitting facile conjugation to proteins, is produced by femtosecond laser ablation of and the nanoparticles (mean size 17 nm) photostable, with long lifetime (1–4 ms) 694 nm emission. With time‐gating complete (>20 dB) suppression cell autofluorescence exogenous fluorophores observed. Nanoparticles imaged in as‐grown cells those immunolabeled quantum dots. Immunoassay binding target biomolecules also demonstrated.