Mechanism of Cellular Uptake to Optimized AuNP Beacon for Tracing mRNA Changes in Living Cells

• DOI: 10.1002/ppsc.201700331
• Publication Date: 2017-12-27T09:31:18Z
• Authors: Siwen Li; Caiping Tian; Yuxi Liu; Zhaohui Wang; Yi Ma; Zhihao Han; Jiaying Du; Jinnan Zhang; Yueqing Gu
• Keywords: Molecular beacon

Abstract Molecular beacon is a promising tool for mRNA detection in living cells. But the low detecting efficiency and narrow application range limited its development. In this study, we synthesized novel gold nanoparticle (AuNP) by optimizing sequence amount modified polyethylene glycol (PEG) cell‐penetrating peptide (CPP) on core. Then, mechanism of cell uptake was investigated. Lastly, used AuNP to study Akt‐mTOR‐HIF‐1 signaling pathway function miR‐7 breast tumor The results showed that optimization obviously amplified fluorescence signal beacon. described process cellular confirmed amplifying could enhance signal. Compared results, accuracy similar real‐time‐Q‐PCR (RT‐PCR) western blotting but operation much simpler. Furthermore, found our Akt had small interfering RNA (siRNA). summary, may be method pathways.