Detection of biofilm in bronchoalveolar lavage from children with non‐cystic fibrosis bronchiectasis

• DOI: 10.1002/ppul.23031
• Publication Date: 2014-03-18T12:35:20Z
• Authors: Robyn L. Marsh; Ruth B. Thornton; Heidi C. Smith‐Va...; Peter Richmond; Susan J. Pizzutto; Anne B. Chang
• Keywords: Paediatrics and Reproductive Medicine; Innate immunity; 0301 basic medicine; 03 medical and health sciences; Otitis-media; Bacterial biofilms; Pseudomonas-aeruginosa biofilms; Public Health and Health Services not elsewhere classified; Haemophilus-influenzae biofilm; Non-CF bronchiectasis; 3. Good health

Summary Background The presence of Pseudomonas aeruginosa biofilms in lower airway specimens from cystic fibrosis (CF) patients is well established. To date, biofilm has not been demonstrated bronchoalveolar lavage (BAL) people with non‐CF bronchiectasis. aim this study was to determine (i) if present BAL children and without bronchiectasis, (ii) detection differed between sequentially collected BAL. Methods Testing for two bronchiectasis done using BacLight™ live–dead staining lectin extracellular polymeric matrices. Bacterial culture cytological measures were performed on the first second lavages, respectively. Clinically important infection defined as >10 4 cfu respiratory pathogens/ml Results Biofilm detected seven eight (87.5%) (aged 0.8–6.9 years), but any three controls 1.3–8.6 years). contained both live dead bacteria irrespective antibiotic use prior bronchoscopy. more frequently than first. Three biofilm‐positive culture‐positive pathogens at clinically levels. Conclusions even when BAL‐defined absent. Studies characterize how contributes disease progression treatment outcomes are necessary. Pediatr Pulmonol. 2015; 50:284–292. © 2014 Wiley Periodicals, Inc.