Potential functionality of Cutibacterium acnes extracellular vesicles in atopic dermatitis and acne vulgaris: A comparative proteomic analysis

• DOI: 10.1002/prca.202300106
• Publication Date: 2024-04-19T15:14:13Z
• Authors: Tianze Yu; Jin Chen; Shi Wu; Min Jiang; Ling Han; Ying Ma
• Keywords: Propionibacterium Acnes; Epidermis (zoology); Human skin

Abstract Background Cutibacterium acnes is a commensal bacterium residing in healthy skin and plays critical role maintaining homeostasis. C. has been considered closely related to acne vulgaris, while recent studies suggest that its metabolites may have protective atopic dermatitis (AD) by modulating the immune system Extracellular vesicles (EVs) are small membranous secreted bacteria participate bacteria‐host interactions. Methods This study first compared EVs from AD lesions (AD‐EVs), (Acne‐EVs), (NC‐EVs), using Label‐free quantitative LC‐MS/MS validated differently expressed proteins parallel reaction monitoring (PRM). Then Normal Human Epidermal Keratinocytes (NHEK) human primary keratinocytes (KC) were treated with isolated different groups, expressions of inflammatory factors measured real‐time PCR Western blotting. Results Compared group, group showed greater downregulation energy metabolism carbon source utilization pathway. Differences protein profile lesion‐separated correspond abnormal sebum secretion pattern both diseases. groups affected Th1 Th2 epidermal barrier markers NHEK KC, indicating immunomodulatory potentials. Conclusions observed distinct proteomic differences between AD‐EVs Acne‐EVs, provided insights into functional acne.