Viral envelope proteins fused to multiple distinct fluorescent reporters to probe receptor binding

• DOI: 10.1002/pro.4974
• Publication Date: 2024-03-27T07:50:50Z
• Authors: Ilhan Tomris; Roosmarijn van de...; Rebeca de Paiva F...; Alba Torrents de ...; Andrew B. Ward; Robert P. de Vries
• Keywords: 0301 basic medicine; 0303 health sciences; receptor-binding; SARS-CoV-2; multivalency; GFP; Biochemistry; Recombinant Proteins; attachment protein; influenza a virus; Viral Envelope Proteins; Polysaccharides; Spike Glycoprotein, Coronavirus; hemagglutinin; Molecular Biology; Research Articles; Protein Binding

Abstract Enveloped viruses carry one or multiple proteins with receptor‐binding functionalities. Functional receptors can be glycans, proteinaceous, both; therefore, recombinant protein approaches are instrumental in attaining new insights regarding viral envelope properties. Visualizing and measuring receptor binding typically entails antibody detection direct labeling, whereas fluorescent fusions attractive tools molecular biology. Here, we report a suite of distinct fusions, both N‐ C‐terminal, for influenza A virus hemagglutinins SARS‐CoV‐2 spike RBD. The contained three six barrels were applied directly to cells assess