CYP9A12andCYP9A17in the cotton bollworm,Helicoverpa armigera: sequence similarity, expression profile and xenobiotic response

• DOI: 10.1002/ps.1832
• Publication Date: 2009-09-02T14:20:11Z
• Authors: Xiaojie Zhou; Caixia Ma; Mei Li; Chengfa Sheng; Huixia Liu; Xinghui Qiu
• Keywords: 0301 basic medicine; 2. Zero hunger; 03 medical and health sciences; Cytochrome P-450 Enzyme System; Gene Expression Profiling; Sequence Homology, Nucleic Acid; Genome, Insect; Molecular Sequence Data; Animals; Amino Acid Sequence; Sequence Analysis, DNA; Moths

BACKGROUND: Helicoverpa armigera (Hübner) causes severe losses in many crops including cotton. Cytochrome P450s play crucial roles the metabolism of important compounds various organisms. The authors attempt to identify new cytochrome P450 genes and investigate their expression profile xenobiotic response order understand nature this pest. RESULTS: A novel cDNA (CYP9A17), encoding a protein 531 amino acid residues, was isolated from H. armigera. CYP9A17 is typical microsomal P450, showing highest (93.9%) identity with CYP9A12 high similarity not only found between cDNAs, but also intron–exon organisation. constitutively universally expressed all four life stages tested larval tissues, whereas specifically midgut fat body. Real-time quantitative RT-PCR showed that level both mRNA affected dose-dependent tissue-specific manners by deltamethrin, gossypol phenobarbital. CONCLUSION: sequence identity, differential patterns, suggesting might diverge via subfunctionalisation after gene duplication event. Copyright © 2009 Society Chemical Industry