Field‐evolved resistance to 11 insecticides and the mechanisms involved in Helicoverpa armigera (Lepidoptera: Noctuidae)

• DOI: 10.1002/ps.6548
• Publication Date: 2021-07-07T09:39:00Z
• Authors: Qinqin Wang; Changhui Rui; Li Wang; Shahzad Ali Nahiyoon; Weiling Huang; Junshu Zhu; Xuejiao Ji; Qingjie Yang; Huizhu Yuan; Li Cui
• Keywords: Indoxacarb; Chlorfenapyr; Abamectin; Carbosulfan; Cyhalothrin; Emamectin

Abstract BACKGROUND To understand the ongoing resistance of cotton bollworm, Helicoverpa armigera , sensitivity five field populations to commonly used insecticides, indoxacarb, abamectin, methoxyfenozide, chlorfenapyr, chlorantraniliprole, spinetoram, lambda‐cyhalothrin, carbosulfan, metaflumizone, chlorpyrifos, and flufenoxuron, were evaluated. Furthermore, biochemical molecular mechanisms field‐evolved in H. also investigated. RESULTS Five showed moderate carbosulfan lambda‐cyhalothrin. The ratio (RR) indoxacarb was significantly correlated with glutathione‐S‐transferases (GSTs) activity ( r = 0.913, P 0.011). Methoxyfenozide RR largely cytochrome P450s 0.860, 0.028). Besides, six genes CYP4L5 AQP, CYP6B7 CYP9A14 HDP BDP, CYP9A17V2 YSP, CYP332A1 HDP, LFP, AQP CYP337B1 two GSTs GSTd1 GSTs1 overexpressed (>5‐fold). Moreover, positively overexpression 0.880, 0.98 0.86, 0.021, 0.001 0.028, respectively). transcript found be metaflumizone 0.950, 0.004) 0.850, 0.033), respectively. CONCLUSION can effectively controlled using chlorpyrifos spinetoram Hebei Shandong provinces. present study demonstrated that relative expression level .