Periplocoside P affects synaptic transmission at the neuromuscular junction and reduces synaptic excitability in Drosophila melanogaster by inhibiting V‐ATPase

• DOI: 10.1002/ps.7705
• Publication Date: 2023-08-09T17:52:45Z
• Authors: Jiaojiao Luo; Anqi Sun; Yang Yu; Yakun Pei; Yayun Zuo; Zhaonong Hu
• Keywords: Neuromuscular transmission

Periplocoside P (PSP) is a major component of Periploca sepium Bunge known for its potent insecticidal activity. V-Type adenosine triphosphatase (V-ATPase), which widely distributed in the cytoplasmic membranes and organelles eukaryotic cells, plays crucial role synaptic excitability conduction. Previous research has shown that PSP targets apical membrane goblet cells insect midgut. However, effects on transmission at neuromuscular junction are often overlooked.The bioassay revealed Drosophila adults with different genetic backgrounds showed varying levels susceptibility to order: parats1 > ;DSC1-/- ≈ w1118 DSC1-/- . Intracellular electrode recording demonstrated PSP, similar bafilomycin A1, had an impact amplitude excitatory potential (EJP) accelerated decay. Furthermore, alteration EJP concentration-dependent. Another surprising discovery was knockout DSC1 channel insensitivity PSP.Our findings confirm can influence larvae by targeting V-ATPase. These results provide basis investigating mechanism action application designing novel insecticides. © 2023 Society Chemical Industry.