Quercetin Up‐regulates LDL Receptor Expression in HepG2 Cells

• DOI: 10.1002/ptr.4646
• Publication Date: 2012-03-05T17:18:50Z
• Authors: Jiyoung Moon; Seung‐Min Lee; Hyun Ju Do; Yoonsu Cho; Ji Hyung Chung; Min‐Jeong Shin
• Keywords: 0301 basic medicine; 03 medical and health sciences; Receptors, LDL; Transcription, Genetic; MAP Kinase Signaling System; JNK Mitogen-Activated Protein Kinases; Humans; Quercetin; Hep G2 Cells; Extracellular Signal-Regulated MAP Kinases; Sterol Regulatory Element Binding Protein 2; Up-Regulation

Quercetin, an abundant flavonol found in fruits and vegetable, has been implicated lowering the risk of cardiovascular disease that is often associated with high plasma levels low density lipoprotein (LDL) cholesterol. Here we investigated whether quercetin could modulate expression LDL receptors (LDLR) HepG2 cells possible underlying mechanisms to exert quercetin's effects. We was able induce LDLR at least a 75 µ m concentration, which accompanied by increase nuclear sterol regulatory element binding protein 2 (SREBP2). This effect mediated activation c‐jun‐N‐terminal kinase (JNK) extracellular signal‐regulated (ERK) signalling pathways as experiments using chemical inhibitors each pathway. When were challenged synthesis quercetin‐activated transcription, mRNA not significantly affected cycloheximide but puromycin abolished quercetin‐induced transcription. Taken together, conclude can initiate transcription enhancing SREBP2 processing, new might be necessary maximum up‐regulation gene. Our findings demonstrate strongly up‐regulated gene expression, elicit hypolipidemic effects increasing clearance circulating cholesterol from blood. Copyright © 2012 John Wiley & Sons, Ltd.