Supplementation with extract of pumpkin seeds exerts estrogenic effects upon the uterine, serum lipids, mammary glands, and bone density in ovariectomized rats

• DOI: 10.1002/ptr.6280
• Publication Date: 2019-01-21T03:40:20Z
• Authors: Beni Lestari; Ziana Walidah; Rohmad Yudi Utomo; Retno Murwanti; Edy Meiyanto
• Keywords: Pumpkin seed

Pumpkin seeds contain lignan phytoestrogens, which have the potential to retrieve menopausal syndromes caused by estrogen deficiency. This study aims determine estrogenic effects of extract pumpkin (EPS). In vitro experiment was performed 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay on CHO‐K1 cells. vivo experiments were conducted using 24 7‐weeks‐old female Sprague Dawley rats, divided into four treatment groups (ovariectomized [OVX], ovariectomized for with 2‐μM 17β‐estradiol, 500 mg/kg BW EPS, and 1000 body weight (BW) EPS) two baseline (OVX sham). Uterine weight, mammary glands, serum lipid, bone density observed after 30 days. silico through molecular docking secoisolariciresinol lariciresinol receptors (ERs). EPS exhibited a biphasic effect, inducing cell growth at 10–100 μg/mL decreasing viability 250–1000 μg/mL. improved uterine retrieved glands comparable 17β‐estradiol. Meanwhile, increased high‐density lipoprotein (HDL), decreased low‐density (LDL), recovered in correlation osteoclasts increasing osteoblasts. The score ERα ERβ −91.13 −95.87, respectively, −80.91 −90.29, respectively. These results demonstrated that performs properties rats model overcome symptoms