Corosolic acid ameliorates non‐alcoholic steatohepatitis induced by high‐fat diet and carbon tetrachloride by regulating TGF‐β1/Smad2, NF‐κB, and AMPK signaling pathways

• DOI: 10.1002/ptr.7195
• Publication Date: 2021-07-02T10:49:26Z
• Authors: Guancheng Liu; Zhe Cui; Xiaoyan Gao; Huizhe Liu; Linghe Wang; Jinyan Gong; Ao Wang; Jianxiu Zhang; Qianqian Ma; Yuan Huang; Guangchun Piao; Haidan Yuan
• Keywords: Steatohepatitis; AMP-Activated Protein Kinase

Hawthorn ( Crataegus pinnatifida Bunge. var. major ) is an edible and medicinal fruit that very common in food traditional Chinese medicine. Corosolic acid (CA), a pentacyclic triterpenoid, which active component of hawthorn ), has been exhibiting various pharmacological activities such as antidiabetic, antibacterial, anticancer, antiinflammatory, antioxidant effects. The study aimed to evaluate the effect CA on non‐alcoholic steatohepatitis (NASH) mice induced by 60 kcal% high‐fat diet (HFD) carbon tetrachloride (CCl 4 ). lowered liver index serum AST, ALT, TG, TC levels compared those model group. Histological analyses tissues treated with revealed significantly decreased number lipid droplets alleviated inflammation fibrosis. inhibited transcripts pro‐fibrogenic markers (including α‐SMA, collagen I, TIMP‐1) pro‐inflammatory cytokines TNF‐α, IL‐1β, caspase‐1, IL‐6) associated hepatic fibrosis, NF‐κB translocation TGF‐β1/Smad2 AMPK pathways. In addition, reduced accumulation via regulation activation FFA‐induced steatotic HepG2 cells. also I expressions, Smad2 phosphorylation, were TGF‐β1 treatment LX2 Our results suggested ameliorated NASH through regulating TGF‐β1/Smad2, NF‐κB, signaling pathways, could be developed potential health functional or therapeutic agent for patients.