Time‐dependent dual beneficial modulation of interferon‐γ, interleukin 5, and Treg cytokines in asthma patient peripheral blood mononuclear cells by ganoderic acid B

• DOI: 10.1002/ptr.7266
• Publication Date: 2022-02-03T13:20:47Z
• Authors: Changda Liu; Mingzhuo Cao; Nan Yang; Jessica Reid‐Adam; Jody Tversky; Jixun Zhan; Xiu‐Min Li
• Keywords: GATA3; Interleukin 13

Th2 cytokines play a dominant role in the pathogenesis of allergic asthma. Interferon gamma (IFN-γ), Th1 cytokine, links to therapeutic mechanisms Interleukin (IL)-10, regulatory is involved induction immune tolerance. We previously demonstrated that Anti-Asthma Simplified Herbal Medicine Intervention (ASHMI) suppressed and increased IFN-γ patients with asthma animal models, but its bioactive compound unknown. Ganoderic acid beta (GAB) was isolated from Ganoderma lucidum (one herb ASHMI). Human peripheral blood mononuclear cells (PBMCs) adult were cultured GAB or dexamethasone (Dex) presence environmental allergens. The cytokine levels IL-10, IFN-γ, IL-5, transcription factors T-bet, Foxp-3, GATA3 measured. Following 3-day culture, GAB, not Dex, significantly IL-10 by patients' PBMCs. 6-day treatment, inhibited IL-5 production, remained high. Dex production all three cytokines. maintained Foxp-3 T-bet gene expression, while expression. simultaneously associated Foxp3, suppressing which suppression GATA3, demonstrating unique beneficial modulatory effect, distinguishes Dex's overall suppression.