The holy basil administration diminishes the NF‐kB expression and protects alveolar epithelial cells from pneumonia infection through interferon gamma

• DOI: 10.1002/ptr.7428
• Publication Date: 2022-03-02T07:09:05Z
• Authors: Arundhathy Suresh; Tejeshwar C. Rao; Sumeet Solanki; Madathilparambil ...; Bindu Menon; Krishnan Raghaven...
• Keywords: Proinflammatory cytokine; Viability assay

Abstract Bacterial pneumonia is one of the most important causes mortality in United States. The bacteria Klebsiella pneumoniae ( KP ) accounts for a significant proportion community and hospital‐acquired infections. Here, we determine that holy basil Ocimum sanctum extract improves cell viability dampens proinflammatory cytokine response an vitro model pneumonia. For this, A549, human alveolar basal epithelial line, was subjected to lethal following 24‐hr pretreatment with extract. Bacteremia, viability, apoptosis, MTT assay, phagocytic capacity, cytokines, Khe gene expression were assessed these cells Cell morphology analysis showed protected A549 from infection–mediated effects by inhibiting death due apoptosis. Additionally, presence basil, demonstrated significantly higher bactericidal capacity phagocytosis. Administration led reduced hypoxia‐inducible factor‐1/2a, nuclear factor kappa B, KP‐ infected while increasing interferon (IFN)‐γ expression. Our results suggest setting infection, likely via attenuation IFN‐γ mediated signaling pathways. Holy promising therapeutic agent managing treating bacterial based on its potency.