Angelica sinensis polysaccharide improves mitochondrial metabolism of osteoarthritis chondrocytes through PPARγ/SOD2/ROS pathways

• DOI: 10.1002/ptr.7979
• Publication Date: 2023-08-26T07:31:02Z
• Authors: Su Ni; Ning Yi; Hang Yuan; Dong Li; Xu Chen; Chao Zhuang
• Keywords: Angelica sinensis; SOD2

Osteoarthritis (OA) is a common degenerative joint disease, which characterized by wear of articular cartilage and narrow space, resulting in movement disorder. At present, accurate molecular mechanisms effective interventions are still being explored. Here, we propose that angelica sinensis polysaccharide (ASP) alleviates OA progression activating peroxisome proliferator-activated receptor gamma (PPARγ). Therapeutic effect ASP improving mitochondrial metabolism chondrocytes was evaluated vitro vivo, respectively. During cell experiments, the concentration time response tert butyl hydroperoxide (TBHP) were determined viability. Apoptosis detected flow cytometry. Mitochondrial reactive oxygen species (ROS), membrane potential (MMP), release cytochrome C, adenosine triphosphate (ATP) production, superoxide dismutase 2 (SOD2) activity. Expressions Aggrecan, collagen type II (Col2a1), PPARγ, SOD2 qRT-PCR western blot. In animal apoptosis target protein expression separately through terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining immunohistochemistry. Pretreatment significantly activated PPARγ rat incubated with TBHP, cleared ROS, improved metabolism, increased viability, alleviated apoptosis. administration could effectively ameliorate degeneration rats, promote extracellular matrix synthesis, decelerate progress OA. Our research identifies role PPARγ/SOD2/ROS pathways, provides new idea for treatment