Gamma‐aminobutyric acid (GABA) can affect physiological processes in preimplantation embryos via GABAA and GABAB receptors

• DOI: 10.1002/rmb2.12528
• Publication Date: 2023-07-19T05:23:52Z
• Authors: Veronika Kovaříková; Alexandra Špirková; Zuzana Šefčíková; Jozef Pisko; Laura Kalatová; Juraj Koppel; Dušan Fabian; Štefan Čikoš
• Keywords: Aminobutyric acid; Gamma-Aminobutyric Acid

Abstract Purpose Several widely used substances (e.g., some therapeutics or food supplements) can act on gamma‐aminobutyric acid (GABA) receptors, and we investigated whether the activation of these receptors could affect preimplantation embryo. Methods Transcripts all GABA receptor subunits selected proteins were examined using quantitative RT‐PCR immunohistochemistry. To analyze effects activation, in vitro culture mouse embryos with natural synthetic ligands was used. Results We detected nine transcripts blastocysts 14 ovulated oocytes. The results this study indicate that ionotropic A be formed from α5, β3, γ3 (or δ, π) subunits, A‐ρ ρ2 metabotropic B1b B2 blastocysts. Supplementing medium at concentrations 2–10 mM specific B agonists (at 10–100 μM) significantly increased proportion dead cells GABA‐induced prevented by pretreatment antagonists. Conclusion negatively via receptors.