Establishment of a 3D spheroid culture system to evaluate the responsiveness of uterine leiomyoma cells to female hormones

• DOI: 10.1002/rmb2.12627
• Publication Date: 2025-01-22T12:30:52Z
• Authors: Takahiro Sakai; Shun Sato; Tetsuro Tamehisa; Hitomi Takasaki; Takuya Kajimura; Isao Tamura; Norihiro Sugino
• Keywords: Spheroid; Histology

Abstract Purpose Uterine leiomyomas (ULMs) are classified into those with and without MED12 mutations (MED12m(+) MED12m(−), respectively). This study was undertaken to establish a culture system evaluate the effect of female hormones on growth ULM cells in each subtype. Methods isolated from MED12m(+) or MED12m(−) tissues were cultured monolayer for 7 days four hormone treatments: estrogen (E) progesterone (P) (E + P), E only (E), P (P), medium (CTRL). They also 3D spheroid above treatments fifth treatment: selective receptor modulator SPRM). The hormonal effects evaluated based cell number, size, histology. Results In cultures, did not cause proliferation either sizes both subtypes significantly larger than CTRL comparable SPRM treatments. Histological staining showed that collagen fibers present spheroids P‐treated groups MED12m(+). Conclusion We established cells.