Update on the electrolytic IVC model for pre‐clinical studies of venous thrombosis

• DOI: 10.1002/rth2.12074
• Publication Date: 2018-01-31T18:24:19Z
• Authors: Olivia R. Palmer; Maxim E. Shaydakov; Joshua P. Rainey; Daniel A. Lawrence; Joan M. Greve; José A. Diaz
• Keywords: Methodological Article; 0301 basic medicine; eim; venous thromboembolism; veins; animal models; 3. Good health; 03 medical and health sciences; electrolysis; Health Sciences; magnetic resonance imaging; fibrinolysis; venous thrombosis; Hematology and Oncology

The electrolytic inferior vena cava model (EIM) is a murine venous thrombosis (VT) that produces non-occlusive thrombus. thrombus forms in the direction of blood flow, as observed patients. EIM valuable for investigations therapeutics due to presence continuous flow. However, equipment used induce original description was expensive and has since been discontinued. Further, fibrinolytic system had not previously studied EIM.We aimed provide an alternative. Additionally, we further characterized through mapping current time dependency resolution dynamics, investigated from acute chronic VT.A voltage converter powered by direct power supply constructed validated, providing added benefit significantly reducing costs. volume dynamics assessed MRI, demonstrating flexibility investigate both pro-thrombotic anti-thrombotic conditions. EIM. Centripetal distribution plasminogen over time, with peak staining at day 6 post induction. Both active circulating activator inhibitor-1 (PAI-1) vein wall gene expression PAI-1 peaked 2, coinciding relative decrease tissue urokinase activator.The VT can now be performed low cost may beneficial system.