Characterization of the Effects of Mesenchymal Stromal Cells on Mouse and Human Islet Function

• DOI: 10.1002/sctm.19-0023
• Publication Date: 2019-05-08T10:11:30Z
• Authors: Ahmed A. Arzouni; Andreia Vargas-Se...; Paramjeet K. Dhadda; Chloe L. Rackham; Guo-Cai Huang; Pratik Choudhary; Aileen J. F. King; Peter M. Jones
• Keywords: Cell therapy

Abstract Islet transplantation has the potential to cure type 1 diabetes, but current protocols are not optimal and there is extensive loss of islet β-cell insulin secretory function during immediate post-transplantation period. Studies using experimental models diabetes have shown that coculture islets with mesenchymal stromal cells (MSCs) prior improves graft function, several variables differed among research groups (e.g., MSCs used treatment conditions). We therefore assessed effects on mouse human by investigating importance tissue source for MSCs, protocol configuration length, effect activated different stimuli. derived from adipose (aMSCs) were most effective at supporting secretion in both islets, a direct contact configuration. Preculture aMSCs enhanced phases glucose-induced further responses non-nutrients carbachol arginine. These required period 48–72 hours dependent activation MSCs. Thus, autologous, adipose-derived minimum 48 before implantation likely be an addition protocols. Stem Cells Translational Medicine 2019;8:935–944