Acute On‐Chip HIV Detection Through Label‐Free Electrical Sensing of Viral Nano‐Lysate

• DOI: 10.1002/smll.201202195
• Publication Date: 2013-02-27T14:12:03Z
• Authors: Hadi Shafiee; Muntasir Jahangir; Fatih Inci; ShuQi Wang; Remington B. M. W...; Francoise F. Giguel; Athe M. N. Tsibris; Daniel R. Kuritzkes; Utkan Demirci
• Keywords: Point of care; Bispecific antibody; Point-of-Care Testing; Lab-on-a-Chip

Abstract Development of portable biosensors has broad applications in environmental monitoring, clinical diagnosis, public health, and homeland security. There is an unmet need for pathogen detection at the point‐of‐care (POC) using a fast, sensitive, inexpensive, easy‐to‐use method that does not require complex infrastructure well‐trained technicians. For instance, Human Immunodeficiency Virus (HIV‐1) acute infection stage been challenging, since current antibody‐based POC technologies are effective due to low concentration antibodies. In this study, we demonstrated first time label‐free electrical sensing can detect lysed viruses, i.e. viral nano‐lysate, through impedance analysis, offering alternative technology methods such as dipsticks Enzyme‐linked Immunosorbent Assay (ELISA). The presented broadly applicable platform potentially be adapted multiple pathogens utilizing spectroscopy other infectious diseases including herpes, influenza, hepatitis, pox, malaria, tuberculosis. offers rapid tool used resource‐constrained settings, well hospital primary care settings.