Smart Hydrogel Microfluidics for Single‐Cell Multiplexed Secretomic Analysis with High Sensitivity

• DOI: 10.1002/smll.201802918
• Publication Date: 2018-10-18T06:50:55Z
• Authors: Myat Noe Hsu; Shih‐Chung Wei; Song Guo; Dinh‐Tuan Phan; Yong Zhang; Chia‐Hung Chen
• Keywords: Single-Cell Analysis

Abstract Secreted proteins determine a range of cellular functionalities correlated with human health and disease progression. Because cell heterogeneity, it is essential to measure low abundant protein secretions from individual cells single‐cell activities. In this study, an integrated platform consisting smart hydrogel immunosensors for the sensitive detection developed. A single microparticles are encapsulated within droplet. After incubation, target secreted captured in particle immunoassay. The temperature‐induced volume phase transition biosensor allows concentration analytes gel matrix increase, enabling high‐sensitivity measurements. Distinct heterogeneity live determined 6000 1 h. This method tested secretions, such as interleukin‐6, interleukin‐8, monocyte chemoattractant protein‐1 both suspended (HL60) adherent (MCF7 MDA‐MB‐231). highly flexible can be used simultaneously wide clinically relevant secretions; thus represents novel tool precise biological assays.