Metal–Organic Cage as Single‐Molecule Carrier for Solid‐State Nanopore Analysis

• DOI: 10.1002/smtd.202200743
• Publication Date: 2022-10-11T02:01:09Z
• Authors: Zhan Wang; Rui Hu; Rui Zhu; Wenlong Lu; Guanghao Wei; Jing Zhao; Zhi‐Yuan Gu; Qing Zhao
• Keywords: Biomolecule; Nanopore; Aptamer

The ability to detect biomolecules at the single-molecule level is forefront of biological research, precision medicine, and early diagnosis. Recently, solid-state nanopore sensors have emerged as a promising technique for label-free precise diagnosis assay. However, insufficient sensitivity selectivity small analytes are great challenge clinical applications via nanopores. Here, first time, metal-organic cage, PCC-57, employed carrier increase nanopores based on intrinsic interaction nanocage with biomolecules. Firstly, it found that itself undetectable unless bound target used oligonucleotides linkers attach PCC-57 analytes. Secondly, two analytes, oligonucleotide conjugated angiopep-2 polyphosphoric acid, successfully distinguished using molecular carrier. Finally, detection achieved by attaching oligonucleotide-tailed aptamers, human alpha-thrombin sample detected. It believed highly designable cage could serve rich repository variety biomolecules, facilitating screening clinically relevant in future.