Polydopamine‐Decorated PLCL Conduit to Induce Synergetic Effect of Electrical Stimulation and Topological Morphology for Peripheral Nerve Regeneration

• DOI: 10.1002/smtd.202200883
• Publication Date: 2023-01-04T02:14:22Z
• Authors: Shunyi Lu; Wen Chen; Jiayi Wang; Zilong Guo; Lan Xiao; Lingyu Wei; Jieqin Yu; Ya Yuan; Weisin Chen; Mengxuan Bian; Lei Huang; Yuanyuan Liu; Jian Zhang; Yu‐Lin Li; Li‐Bo Jiang
• Keywords: Nerve guidance conduit; Electrical conduit; Peripheral nerve injury

Due to the limited self-repairing capacity after peripheral nerve injuries (PNI), artificial conduits are widely applied facilitate neural regeneration. Exogenous electrical stimulation (ES) that is carried out by conductive conduit regulates biological behavior of Schwann cells (SCs). Meanwhile, a longitudinal surface structure counts guide axonal growth accelerate end-to-end connection. Currently, there no equipped with both conduction and axon-guiding structure. Herein, biodegradable, poly(l-lactide-co-caprolactone)/graphene (PLCL/GN) composite designed. The 20.96 ± 1.26 MPa tensile strength has micropatterned 20 µm groove fabricated microimprint technology self-assembled polydopamine (PDA). In vitro evaluation shows ES effectively stimulate directional cell migration, adhesion, elongation, enhance neuronal expression SCs. rat sciatic crush model demonstrates promotes myelin sheath, faster regeneration, 20-fold functional recovery in vivo. These discoveries prove PLCL(G)/PDA/GN promising tool for PNI treatment providing integration physical guidance, biomimetic regulation, bioelectrical stimulation, which inspires novel therapeutic approach regeneration future.