Electro‐Optical Detection of Single Molecules Based on Solid‐State Nanopores

• DOI: 10.1002/sstr.202000003
• Publication Date: 2020-08-04T07:49:07Z
• Authors: Wenlong Lu; Rui Hu; Xin Tong; Dapeng Yu; Qing Zhao
• Keywords: Nanopore

Electrical detection of single molecule based on solid‐state nanopores is recently combined with optical methods. The synergistic effect the electrical and measurements makes combination powerful. It promising for next‐generation single‐molecule approach. Conventional nanopore sensors monitor pulses caused by molecules translocating through nanopore. However, signal limited insufficient resolutions, low throughput, lack specificity, manipulation. Optical has been extensively studied since past decades. signals, such as surface‐enhanced Raman spectroscopy (SERS) fluorescence, have unique sensitivity specificity molecules. Recently, methods are developed to complement limitations nanopores. This review describes main approaches used electro‐optical nanopores, including total internal reflection fluorescence (TIRF), confocal microscopy, plasmonic nanostructures. Laser‐induced heating, trapping, strategies reduce background also discussed. Moreover, recent experiments DNA other analytes introduced.