Human Placenta-Derived Mesenchymal Stem Cells Promote Hepatic Regeneration in CCl4-Injured Rat Liver Model via Increased Autophagic Mechanism

• DOI: 10.1002/stem.1396
• Publication Date: 2013-04-17T13:26:54Z
• Authors: Jieun Jung; Jong Ho Choi; Youjin Lee; Jong-Wan Park; Il-Hoan Oh; Seong-Gyu Hwang; Kwang-Soo Kim; Gi Jin Kim
• Keywords: Regenerative Medicine; Liver Regeneration

Mesenchymal stem cells (MSCs) have great potential for cell therapy in regenerative medicine, including liver disease. Even though ongoing research is dedicated to the goal of bringing MSCs clinical applications, further understanding complex underlying mechanisms required. Autophagy, a type II programmed death, controls cellular recycling through lysosomal system damaged or tissues. However, it still unknown whether can trigger autophagy enhance regeneration and/or provide therapeutic effect as survival promoters. We therefore investigated autophagy's activation carbon tetrachloride (CCl4 )-injured rat following transplantation with chorionic plate-derived (CP-MSCs) isolated from placenta. The expression markers apoptosis, autophagy, survival, and were analyzed. Whereas caspase 3/7 activities reduced (p < .05), levels hypoxia-inducible factor-1α (HIF-1α) factors significantly increased by CP-MSCs transplantation. Decreased necrotic .05) autophagic signals .005) observed CCl4 -treated primary hepatocytes during vitro coculture CP-MSCs. Furthermore, upregulation HIF-1α promotes hepatic an mechanism marked light chain 3 (LC 3II). These results suggest that administration repair systemically concomitant involving autophagy. findings involved these processes will help develop new cell-based strategies medicine