Adipose-Derived Mesenchymal Stem Cells Ameliorate Chronic Experimental Autoimmune Encephalomyelitis

• DOI: 10.1002/stem.194
• Publication Date: 2009-08-13T05:44:06Z
• Authors: Gabriela Constantin; Silvia Marconi; Barbara Rossi; Stefano Angiari; Laura Calderan; Elena Anghileri; Beatrice Gini; Simone Dorothea Bach; Marianna Martinello; Francesco Bifari; Mirco Galiè; Ermanna Turano; Simona Budui; Andrea Sbarbati; Mauro Krampera; Bruno Bonetti
• Keywords: Remyelination; Stem Cell Therapy; Encephalomyelitis

Abstract Mesenchymal stem cells (MSCs) represent a promising therapeutic approach for neurological autoimmune diseases; previous studies have shown that treatment with bone marrow-derived MSCs induces immune modulation and reduces disease severity in experimental encephalomyelitis (EAE), an animal model of multiple sclerosis. Here we show intravenous administration adipose-derived (ASCs) before onset significantly the EAE by decreases spinal cord inflammation demyelination. ASCs preferentially home into lymphoid organs but also migrates inside central nervous system (CNS). Most importantly, chronic established ameliorates course both demyelination axonal loss, Th2-type cytokine shift T cells. Interestingly, relevant subset expresses activated α4 integrins adheres to inflamed brain venules intravital microscopy experiments. Bioluminescence imaging shows control ASC accumulation CNS. Importantly, found cultures produce basic fibroblast growth factor, brain-derived platelet-derived factor-AB. Moreover, infiltration within demyelinated areas is accompanied increased number endogenous oligodendrocyte progenitors. In conclusion, clear potential bimodal mechanism, suppressing response early phases as well inducing local neuroregeneration progenitors animals disease. Overall, our data suggest valuable tool cell–based therapy inflammatory diseases