Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference

• DOI: 10.1002/stem.2433
• Publication Date: 2016-06-23T01:23:06Z
• Authors: Wei Yao; Yu-An Evan Lay; Alexander Kot; Ruiwu Liu; Hongliang Zhang; Haiyan Chen; Kit Lam; Nancy E. Lane
• Keywords: Mobilization

Abstract Mesenchymal stem cell (MSC) transplantation has been tested in animal and clinical fracture studies. We have developed a bone-seeking compound, LLP2A-Alendronate (LLP2A-Ale) that augments MSC homing to bone. The purpose of this study was determine whether treatment with LLP2A-Ale or combination MSCs would accelerate bone healing mouse closed model if the effects are sex dependent. A right mid-femur induced two-month-old osterix-mCherry (Osx-mCherry) male female reporter mice. mice were subsequently treated placebo, (500 μg/kg, IV), derived from wild-type Osx-mCherry adipose tissue (ADSC, 3 x 105, IV) ADSC + LLP2A-Ale. In phosphate buffered saline-treated mice, females had higher systemic surface-based formation than males. However, formed larger callus volumetric mineral density strength females. increased exogenous gaps, enhanced incorporation these cells into formation, stimulated endochondral formation. Additionally, engraftment gaps seemed contribute overall improved strength. These sex-independent. There sex-difference rate healing. superior on which independent sex. Increased mobilization sites accelerated regeneration.