Implantation of Stromal Vascular Fraction Progenitors at Bone Fracture Sites: From a Rat Model to a First-in-Man Study

• DOI: 10.1002/stem.2478
• Publication Date: 2016-08-19T09:03:26Z
• Authors: Franziska Saxer; Arnaud Scherberich; Atanas Todorov; Patrick Studer; Sylvie Miot; Simone Schreiner; Sinan Güven; Laurent A.H. Tchang; Martin Haug; Michael Heberer; Dirk J. Schaefer; Daniel Rikli; Ivan Martin; Marcel Jakob
• Keywords: Stromal vascular fraction; Bone cell

Abstract Stromal Vascular Fraction (SVF) cells freshly isolated from adipose tissue include osteogenic- and vascular-progenitors, yet their relevance in bone fracture healing is currently unknown. Here, we investigated whether human SVF directly contribute to the repair of experimental fractures nude rats, explored feasibility/safety clinical use for augmentation upper arm elderly individuals. Human were loaded onto ceramic granules within fibrin gel implanted critical rat femoral after locking-plate osteosynthesis, with cell-free grafts as control. After 8 weeks, only SVF-treated did not fail mechanically displayed formation ossicles at site, vascular structures formed by cells. The same materials combined autologous then used treat low-energy proximal humeral patients (64-84 years old) along standard open reduction internal fixation. Graft manufacturing implantation compatible intraoperative settings led no adverse reactions, thereby verifying feasibility/safety. Biopsies up 12 months, upon plate revision or removal, demonstrated ossicles, structurally disconnected morphologically distinct osteoconducted bone, suggesting osteogenic nature We demonstrate that cells, without expansion exogenous priming, can spontaneously form vessel a fracture-microenvironment. gained insights into biological functionality grafts, facile, intra-operative modality, warrant further tests effectiveness larger, controlled trials.