Single-Cell Transcriptomic Sequencing Analyses of Cell Heterogeneity During Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells

• DOI: 10.1002/stem.3442
• Publication Date: 2021-11-11T09:59:27Z
• Authors: Rongmei Qu; Kai He; Tingyu Fan; Yuchao Yang; Liyao Mai; Zhiwei Lian; Zhitao Zhou; Yan Peng; Asmat Ullah Khan; Bing Sun; Xiaolan Huang; Jun Ouyang; Xinghua Pan; Jingxing Dai; Wenhua Huang
• Keywords: 0303 health sciences; 03 medical and health sciences; Adipose Tissue; Osteogenesis; Humans; Cell Differentiation; Mesenchymal Stem Cells; Transcriptome; Cells, Cultured

Abstract Mesenchymal stem cells (MSCs) are known for their multilineage differentiation potential with immune-modulatory properties. The molecular underpinnings of remain largely undefined. In this study, we investigated the cellular and features chemically induced osteogenesis from MSC isolated human adipose tissue (human MSCs, hAMSCs) using single-cell RNA-sequencing (scRNA-seq). We found that a near complete osteogenic clusters hAMSCs under directional induction. Both groups heterogeneous, some intrinsically prepared osteogenesis, while variant OS seems in cooperation due division general function. identified set genes related to cell stress response highly expressed during differentiation. also characterized series transitional transcriptional waves throughout process osteoblast specified unique gene networks epigenetic status as key markers osteogenesis.