Exploring miR‐21 as a key regulator in two distinct approaches of bone marrow stromal cells differentiation into Schwann‐like cells

• DOI: 10.1002/syn.22293
• Publication Date: 2024-05-23T10:09:12Z
• Authors: Yu‐Mei Liu; He‐Ying Wang; Cai‐Hong Wei; Jun‐Ping Li; Ying Wang; Wen‐Zhi Ma; Hua Jia
• Keywords: 0301 basic medicine; Intracellular Signaling Peptides and Proteins; Membrane Proteins; Cell Differentiation; Mesenchymal Stem Cells; Apoptosis; Nerve Tissue Proteins; Rats; Rats, Sprague-Dawley; MicroRNAs; 03 medical and health sciences; Culture Media, Conditioned; Animals; Schwann Cells; Nerve Growth Factors; Cells, Cultured; Cell Proliferation

Abstract The differentiation of bone marrow stromal cells (BMSCs) into Schwann‐like (SCLCs) has the potential to promote structural and functional restoration injured axons. However, optimal induction protocol its underlying mechanisms remain unclear. This study aimed compare effectiveness different protocols in promoting rat BMSCs SCLCs explore their mechanisms. were induced using two distinct methods: a composite factor approach (Protocol‐1) conditioned culture medium (Protocol‐2). expression Schwann (SCs) marker proteins neurotrophic factors (NTFs) differentiated was assessed. Cell proliferation apoptosis also measured. During induction, changes miR‐21 Sprouty RTK signaling antagonist 2 (SPRY2) mRNA analyzed. Following transfection with agomir or antagomir, carried out both protocols, SPRY2, ERK1/2, SCs examined. results revealed that NTFs higher Protocol‐1, whereas did not significantly differ between groups. Compared Protocol‐2 exhibited enhanced cell fewer apoptotic necrotic cells. Both showed negative correlation SPRY2 throughout stages. After group reduced expression, increased ERK1/2 elevated proteins. demonstrates Protocol‐1 yields stronger proliferation. Upregulating suppresses activates pathway, promotes BMSC SCLCs.