Magnitude‐dependent and inversely‐related osteogenic/chondrogenic differentiation of human mesenchymal stem cells under dynamic compressive strain

• DOI: 10.1002/term.2332
• Publication Date: 2016-09-30T02:08:25Z
• Authors: Christopher B. Ho...; Koji Hirota; Junze Liu; Maricela Maldonado; B. Hyle Park; Jin Nam
• Keywords: Chondrogenesis; RUNX2; SOX9; Strain (injury); mechanobiology

Biomechanical forces have been shown to significantly affect tissue development, morphogenesis, pathogenesis and healing, especially in orthopaedic tissues. Such biological processes are critically related the differentiation of human mesenchymal stem cells (hMSCs). However, mechanistic details regarding how mechanical direct MSC subsequent formation still elusive. Electrospun three-dimensional scaffolds were used culture subject hMSCs various magnitudes dynamic compressive strains at 5, 10, 15 or 20% (ε = 0.05, 0.10, 0.15, 0.20) a frequency 1 Hz for 2 h daily up 28 days osteogenic media. Gene expression chondrogenic markers (ACAN, COL2A1, SOX9) glycosaminoglycan (GAG) synthesis upregulated response increased strain, whereas (COL1A1, SPARC, RUNX2) calcium deposition had noticeable decreases by loading magnitude-dependent manner. Dynamic analysis showed enhanced viscoelastic modulus with respect strain peaking 15%, which coincides maximal GAG synthesis. Furthermore, polarization-sensitive optical coherence tomography revealed that alignment extracellular matrix greatest level 15% as well. Overall, we show degree towards lineage is inversely related, it depends on magnitude strain. These results demonstrate multiphenotypic can be controlled varying regimens, providing novel strategy modulate specification morphogenesis. Copyright © 2016 John Wiley & Sons, Ltd.