Relationship between intracellular Ca2+ and ROS during fluoride‐induced injury in SH‐SY5Y cells

• DOI: 10.1002/tox.20721
• Publication Date: 2011-07-24T17:53:35Z
• Authors: Zhixia Xu; Bayi Xu; Tao Xia; Weihong He; Ping Gao; Lijuan Guo; Zhenglun Wang; Qiang Niu; Aiguo Wang
• Keywords: EGTA; BAPTA; SH-SY5Y; Sodium fluoride

Abstract The mechanisms underlying the neurotoxicology of endemic fluorosis still remain obscure. To explore lactate dehydrogenase (LDH) leakage, intracellular Ca 2+ concentration ([Ca ] i ) and reactive oxygen species (ROS) production induced by fluoride, human neuroblastoma (SH‐SY5Y) cells were incubated with sodium fluoride (NaF, 20, 40, 80 mg/L) for 24 h, 40 mg/L NaF 3, 6, 12, 18, N ‐acetyl‐ L ‐cysteine (NAC), ethyleneglycol‐bis‐(β‐aminoethyl ether)‐ , ′, ′‐tetraacetic acid (EGTA), 1,2‐bis( O ‐aminophenoxy)ethane‐ tetra(acetoxymethyl) ester (BAPTA‐AM) alone or combined (40 respectively 12 h in vitro . results showed that LDH levels fluoride‐treated groups significantly higher than control group (in test level 0.05, difference statistical significance). [Ca ROS reached a peak at 3 after exposure to fluoride. Fluoride coincubated NAC (antioxidant) dramatically decreased compared only However, fluoride‐induced increase was not affected NAC. BAPTA‐AM (intracellular calcium chelator) markedly lowered while EGTA (extracellular have no effects on them. These indicate fluoride‐related release from site storage causes elevation contributing cytotoxicity SH‐SY5Y cells. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2013.