Enniatin B and beauvericin are common in Danish cereals and show high hepatotoxicity on a high‐content imaging platform

• DOI: 10.1002/tox.22367
• Publication Date: 2016-09-15T08:13:18Z
• Authors: Terje Svingen; Niels Lund Hansen; Camilla Taxvig; Anne Marie Vinggaard; Udo Jensen; Peter Have Rasmussen
• Keywords: Beauvericin; Citrinin

Mycotoxins are fungi-born metabolites that can contaminate foods through mould-infected crops. They a significant food/feed-safety issue across the globe and represent substantial financial burden for world economy. Moreover, with changing climate fungal biota, there is now much discussion about emerging mycotoxins measurable at levels in crops world-wide. Unfortunately, we still know very little bioavailability toxic potentials of many these less characterized mycotoxins, including large family enniatins. In this study, present new occurrence data enniatin A, A1, B, B1 beauvericin four Danish crops: oat, wheat, barley from 2010 harvest, rye 2011 harvest. The enniatins were B > A1 A. Enniatin was detected 100% tested samples regardless crop type. addition to data, report proof-of-concept study using human-relevant high-content hepatotoxicity, or "quadroprobe," assay screen their cytotoxic potential. sensitive most compounds 0.009-100 µM range. Among eight (enniatin beauvericin, altenariol, deoxynivalenol, aflatoxin B1, andrastin citrinin, penicillic acid), showed cytotoxicity concentration lower than which archetypal acute hepatotoxic liver-carcinogenic mycotoxin. Hence, quadroprobe hepatotoxicity may become valuable assessment tool toxicity future. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1658-1664, 2017.