Oxidative stress as a damage mechanism in porcine cumulus‐oocyte complexes exposed to malathion during in vitro maturation

• DOI: 10.1002/tox.22384
• Publication Date: 2017-02-10T06:16:44Z
• Authors: Diana Flores; Verónica Souza; Miguel Betancourt; Mario Teteltitla; Humberto González...; Eduardo Casas; Edmundo Bonilla; Patricia Ramírez‐...; María Concepción ...; Yvonne Ducolomb
• Keywords: TBARS; Trolox; Glutathione reductase

Malathion is one of the most commonly used insecticides. Recent findings have demonstrated that it induces oxidative stress in somatic cells, but there are not enough studies this effect germ cells. impairs porcine oocyte viability and maturation, shown how damages maturation which biochemical mechanisms affected process cumulus-oocyte complexes (COCs). The aims present study were to determine amount produced by malathion COCs matured vitro, define affect process, whether trolox can attenuate damage. Sublethal concentrations 0, 750, 1000 µM evaluate antioxidant enzyme expressions, reactive oxygen species (ROS production), protein oxidation, lipid peroxidation, among other oxidation products. decreased a concentration-dependent manner. increased Cu, Zn superoxide dismutase (SOD1), glutathione-S-transferase (GST), glucose 6 phosphate dehydrogenase (G6PD) level glutathione peroxidase (GSH-Px) catalase (CAT) level. Species reactives (ROS), Thiobarbituric acid substances (TBARS) levels exposed insecticide, when pre-treated with (50 µM) 30 min before during exposure, these parameters down control levels. This showed has detrimental on vitro inducing stress, while attenuated toxicity decreasing