CircKDM4C upregulates P53 by sponging hsa‐let‐7b‐5p to induce ferroptosis in acute myeloid leukemia

• DOI: 10.1002/tox.23126
• Publication Date: 2021-03-18T07:49:28Z
• Authors: Li‐hua Dong; Jing‐jing Huang; Peng Zu; Jing Liu; Xue Gao; Jian‐wei Du; Yu‐fu Li
• Keywords: Chromatin immunoprecipitation; Cell Sorting

To investigate the role of circKDM4C in acute myeloid leukemia (AML), expression circKDM4C, hsa-let-7b-5p, and P53 was measured by qRT-RCR. AML cell lines(K-562 HL-60) were transfected correspondingly investigated for proliferation, migration, invasion abilities CCK-8, colony formation, transwell, wound healing assays, respectively. The levels P53, ACSL4, PTGS2, GPX4, FTH1 K-562, HL-60 cells western blotting. Also, mediated regulation ferroptosis studied. Phen Green SK probe confocal laser scanning microscope used to assess cellular iron levels. reactive oxygen species analyzed fluorescence-activated sorting using C11-BODIPY probe. Bioinformatics analysis predicted putative binding sites among P53. These verified dual-luciferase reporter assay, RNA pull-down, immunoprecipitation assays. Finally, vitro findings also vivo nude mice. CircKDM4C significantly down-regulated patients. overexpression lines inhibited invasion, promoted ferroptosis. We found that acts as a sponge hsa-let-7b-5p thereby regulates p53 which is target gene hsa-let-7b-5p. are negatively correlated, while positively correlated Moreover, we induces sponging upregulates This work emphasizes patients, could be explored therapeutic role.