Kynurenine 3-Hydroxylase in Brain: Species Activity Differences and Effect of Gerbil Cerebral Ischemia
Male
0303 health sciences
Brain
Middle Aged
Kidney
Macaca mulatta
Mitochondria
Mixed Function Oxygenases
3. Good health
Intestines
Mice, Inbred C57BL
Kinetics
Mice
03 medical and health sciences
Kynurenine 3-Monooxygenase
Liver
Ischemic Attack, Transient
Organ Specificity
Animals
Humans
Female
Gerbillinae
Lung
DOI:
10.1006/abbi.1993.1567
Publication Date:
2002-09-16T09:50:03Z
AUTHORS (6)
ABSTRACT
We have developed a rapid and highly sensitive assay for brain kynurenine 3-hydroxylase activity. The present study determined some characteristics and species differences of kynurenine 3-hydroxylase activity in brain and evaluated the response of this enzyme to cerebral ischemia. The kynurenine 3-hydroxylase assay is based on the conversion of L-kynurenine to 3-hydroxykynurenine in vitro and the quantification of 3-hydroxykynurenine by high-performance liquid chromatography. Kynurenine 3-hydroxylase activity was detected in human, macaque, rat, mouse, and gerbil brain. Regional gerbil brain activities ranged from 20 to 50 nmol/g/h, while kynurenine 3-hydroxylase activities in other species were one order of magnitude lower. Kynurenine 3-hydroxylase was also detected in lung, kidney, spleen, intestine, and liver of gerbils, with activities larger than in brain. Delayed increases in the activity of kynurenine 3-hydroxylase occur in several brain regions following transient ischemia in gerbils. These changes are particularly marked in regions that showed the most extensive brain damage (hippocampus and striatum). Kynurenine 3-hydroxylase may have an important role in determining the flux of kynurenine in brain.
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