Colorimetric Assay Method for Determination of the Tannin Acyl Hydrolase (EC 3.1.1.20) Activity

• DOI: 10.1006/abio.2001.5185
• Publication Date: 2003-05-19T12:51:21Z
• Authors: K.C. Mondal; D. Banerjee; M. Jana; B.R. Pati
• Keywords: 0106 biological sciences; 0301 basic medicine; 03 medical and health sciences; Molecular Structure; Reproducibility of Results; Colorimetry; Spectrophotometry, Ultraviolet; Carboxylic Ester Hydrolases; 01 natural sciences; Hydrolyzable Tannins

A new colorimetric method of tannase (tannin acyl hydrolase, EC 3.1.1.20) assay has been developed using its specific substrate tannic acid. It is based on the changes in optical density of substrate tannic acid after enzymatic reaction at 530 nm. The residual tannic acid was measured by a modified BSA precipitation method. This assay is very simple, reproducible, and very convenient, and with it tannase activity can be measured in relation to the growth of the organism.