Increased Activity and Intranuclear Expression of Phospholipase D2 in Human Renal Cancer

• DOI: 10.1006/bbrc.2000.3719
• Publication Date: 2002-09-16T14:49:19Z
• Authors: Yutong Zhao; Hidetoshi Ehara; Yukihiro Akao; Masayo Shamoto; Yoshihito Nakagawa; Yoshiko Banno; Takashi Deguchi; Nobuko Ohishi; Kunio Yagi; Yoshinori Nozawa
• Keywords: Cell Nucleus; Male; 0301 basic medicine; Dose-Response Relationship, Drug; Blotting, Western; Middle Aged; Kidney; Immunohistochemistry; Kidney Neoplasms; 3. Good health; 03 medical and health sciences; COS Cells; Phospholipase D; Animals; Humans; Electrophoresis, Polyacrylamide Gel; Female; Adenocarcinoma, Clear Cell; Aged; Oleic Acid

We examined the PLD activities of human renal cancers and found that the PLD2 activity was greatly elevated in almost all cases examined as compared with the adjacent normal region. Western blot analysis showed the increased levels of PLD2 protein, but the PLD1 was not discernible. The oleate-dependent PU) activity was very low but appeared to increase in most cases. Interestingly, the immunohistochemical observations indicated the high expression of PLD2 in the nuclei of clear carcinoma cells. This is the first demonstration which suggests the possible involvement of PLD2 in tumorigenesis of renal cancer.