FGF-10 Is a Chemotactic Factor for Distal Epithelial Buds during Lung Development

• DOI: 10.1006/dbio.1998.8994
• Publication Date: 2002-09-16T15:45:48Z
• Authors: William Y. Park; Barbara Miranda; Djamel Lebeche; Gakuji Hashimoto; Wellington V. Car...
• Keywords: Mesenchyme; Budding

Fibroblast growth factor (FGF) signaling is required for normal epithelial branching in the respiratory system of several species. Recent studies have shown that FGF-10 may be a key regulator lung morphogenesis, based on its pattern expression early and ability to induce buddingin vitro.In this study we investigate whether able direct buds proper positions during development . We maintained localized high levels cultured lungs using FGF-10-soaked heparin beads. exerts powerful chemoattractant effect distal but not proximal epithelium. Epithelial grow toward an source within 24 h, subsequently form concentric layers epithelium around bead. BrdU incorporation analysis suggests FGF-10, contrast FGF-7, modest proliferation In absence mesenchyme requires associated proliferative signal bud migration. This can provided by extract from mesenchyme, or also present embryonic lung. does seem interfere with cell differentiation. The reminiscent patterning theDrosophilaFGF ortholog branchless developing tracheal epithelium, suggesting function these genes has been conserved evolution.