Induced Pluripotent Stem Cells from Nonhuman Primates

• DOI: 10.1007/7651_2014_159
• Publication Date: 2014-12-24T21:40:29Z
• Authors: Anuja Mishra; Zhifang Qiu; Steven L. Farnsworth; Jacob J. Hemmi; Miao Li; Alexander V. Pick...; Peter J. Hornsby
• Keywords: Mice, Knockout; Primates; 0301 basic medicine; SOXB1 Transcription Factors; Genetic Vectors; Induced Pluripotent Stem Cells; Cell Culture Techniques; Genes, myc; Kruppel-Like Transcription Factors; Callithrix; Fibroblasts; Cellular Reprogramming; 3. Good health; Kruppel-Like Factor 4; Mice; Proto-Oncogene Proteins c-myb; 03 medical and health sciences; Retroviridae; Animals; Humans; Cellular Reprogramming Techniques; Octamer Transcription Factor-3; Cells, Cultured

Induced pluripotent stem cells from nonhuman primates (NHPs) have unique roles in cell biology and regenerative medicine. Because of the relatedness of NHPs to humans, NHP iPS cells can serve as a source of differentiated derivatives that can be used to address important questions in the comparative biology of primates. Additionally, when used as a source of cells for regenerative medicine, NHP iPS cells serve an invaluable role in translational experiments in cell therapy. Reprogramming of NHP somatic cells requires the same conditions as previously established for human cells. However, throughout the process, a variety of modifications to the human cell protocols must be made to accommodate significant species differences.