Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration

• DOI: 10.1007/7651_2018_135
• Publication Date: 2018-05-07T07:02:40Z
• Authors: Ehsan Taghiabadi; Bahareh Beiki; Nasser Aghdami; Amir Bajouri
• Keywords: Skin, Artificial; Wound Healing; Fetus; Tissue Engineering; Pregnancy; Humans; Regeneration; Wounds and Injuries; Female; Amnion; Fibroblasts; Cells, Cultured

Various natural and synthetic biomaterials have been applied as skin substitutes for regenerating damaged skin. Here, we describe a straightforward method for fabrication of a tissue-engineered skin substitute by seeding human fetal fibroblasts on acellular human amniotic membrane (HAM). Fetal fibroblasts are achieved from the skin of normal and non-macerated fetus of 11-14 weeks old after spontaneous pregnancy termination. Acellular HAM is obtained by separation of the outer membrane of the chorion and removing its epithelial cells.