Generation of Human Blastoids from Naive Pluripotent Stem Cells

• DOI: 10.1007/7651_2023_485
• Publication Date: 2023-06-24T09:41:53Z
• Authors: Yulei Wei; Kun Liu; Carlos A. Pinzon-...; Deirdre Logsdon; Leqian Yu; Ye Yuan; Jun Wu

Under certain culture conditions, naive human pluripotent stem cells can generate human blastocyst-like structures (called human blastoids). Human blastoids serve as an accessible model for human blastocysts and are amenable for large-scale production. Here, we describe a detailed step-by-step protocol for the robust and high-efficient generation of human blastoids from naive human pluripotent stem cells.