Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells

• DOI: 10.1007/978-1-0716-0146-4_6
• Publication Date: 2019-11-09T08:02:45Z
• Authors: Aline Do Minh; Michelle Yen Tran; Amine A. Kamen
• Keywords: Transduction (biophysics); HEK 293 cells; Suspension culture

The production of lentiviral vectors (LVs) in human embryonic kidney 293 (HEK293) cells using serum-free medium in a suspension culture for the transduction of chimeric antigen receptor T-cells (CAR-T) can be achieved by different methods. This chapter describes LV production by transient transfection, induction of stable packaging cell lines, and induction of stable producer cell lines.