Biolistic Transformation of Japonica Rice Varieties

• DOI: 10.1007/978-1-0716-0356-7_8
• Publication Date: 2020-04-10T08:02:31Z
• Authors: Raviraj Banakar; Kan Wang
• Keywords: 0301 basic medicine; 2. Zero hunger; 0303 health sciences; Osmosis; Inheritance Patterns; Sterilization; Oryza; Biolistics; Plants; 03 medical and health sciences; Transformation, Genetic; Seeds; Regeneration; Gold; Transgenes; Plasmids

Genetic improvement of rice is crucial to achieve global food security as rice is an important staple crop for more than half of the global population. One of the methodologies for genetic improvement is biolistic delivery of genetic components into plant cells. In this chapter, we describe steps involved in introducing plasmid DNA carrying gene of interest into rice mature embryos using Biolistic® PDS-1000/He particle delivery system. We also provide information required for recovery of transformed plants and production of transgenic seed for next generation analysis. Using this protocol, typical 50-70 putative independent transgenic callus lines can be generated from 100 bombarded embryos. Transgenic rice plantlets can be produced within 2 months after the initiation of seed germination for transformation.