Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture

• DOI: 10.1007/978-1-0716-0763-3_4
• Publication Date: 2020-07-17T16:50:23Z
• Authors: Alexey I. Chizhik; Anna M. Chizhik; Daja Ruhlandt; Janine Pfaff; Narain Karedla; Ingo Gregor; Ralph H. Kehlenbach; Jörg Enderlein
• Keywords: Cell Nucleus; DNA-Binding Proteins; Nuclear Pore Complex Proteins; Imaging, Three-Dimensional; Microscopy, Fluorescence; Nuclear Envelope; Nuclear Pore; Humans; Membrane Proteins; 7. Clean energy; HeLa Cells; Molecular Chaperones

The nuclear envelope, comprising the inner and the outer nuclear membrane, separates the nucleus from the cytoplasm and plays a key role in cellular functions. Nuclear pore complexes (NPCs) are embedded in the nuclear envelope and control transport of macromolecules between the two compartments. Recently, it has been shown that the axial distance between the inner nuclear membrane and the cytoplasmic side of the NPC can be measured using dual-color metal-induced energy transfer (MIET). This chapter focuses on experimental aspects of this method and discusses the details of data analysis.