Assessment of Pericyte Phenotype by Flow Cytometry

• DOI: 10.1007/978-1-0716-1056-5_3
• Publication Date: 2021-02-12T06:03:06Z
• Authors: Nusrat S. Khan; Christopher C. West; Fiona Rossi; Mihaela Crisan
• Keywords: Pericyte; Cell Sorting; Mural cell; Cytometry

Pericytes are mural cells closely associated with endothelial cells in capillaries and microvessels. They are precursors of mesenchymal stem/stromal cells that have historically been retrospectively characterized in culture. We established a protocol, described in this chapter, to characterize and isolate pericytes from multiple human organs by flow cytometry and fluorescence-activated cell sorting. This prospective purification of pericytes brings us a step forward in the development of strategies for their use in the clinic.