Analysis of Protein–Glycosaminoglycan Interactions Using Traveling Wave Ion-Mobility Mass Spectrometry

• DOI: 10.1007/978-1-0716-1398-6_8
• Publication Date: 2021-10-10T17:22:57Z
• Authors: Robert V. Williams; I. Jonathan Amster
• Keywords: Ion-mobility spectrometry; Biomolecule; Ion-mobility spectrometry–mass spectrometry; Envelope (radar)

Traveling wave ion-mobility mass spectrometry (TWIMS) combined with native mass spectrometry (MS) has emerged as a powerful tool for analyzing biomolecules, including complexes of protein and heparan sulfate (HS). This technique allows determination of the stoichiometry of the protein-HS interaction and information on the overall 3D molecular envelope.