Analysis of Protein–Glycosaminoglycan Interactions Using Traveling Wave Ion-Mobility Mass Spectrometry

• DOI: 10.1007/978-1-0716-1398-6_8
• Publication Date: 2021-10-10T17:22:57Z
• Authors: Robert V. Williams; I. Jonathan Amster
• Keywords: 0301 basic medicine; 03 medical and health sciences; Ion Mobility Spectrometry; Proteins; 01 natural sciences; Mass Spectrometry; 0104 chemical sciences; Glycosaminoglycans

Traveling wave ion-mobility mass spectrometry (TWIMS) combined with native mass spectrometry (MS) has emerged as a powerful tool for analyzing biomolecules, including complexes of protein and heparan sulfate (HS). This technique allows determination of the stoichiometry of the protein-HS interaction and information on the overall 3D molecular envelope.